HGH destroyed during reconstitution?

[caret912]

I'm looking to see if anyone has heard of hgh being rendered inert by pushing the water in too fast while reconstituting/shaking the vial. I'm also curious if high heat during transportation can damage hgh if it hasn't been reconstituted yet.

I sprayed the water all over the peptide and then shook the vial to get it to dissolve. Minutes later I read an article that said to not do any of that stuff as it will destroy the peptide. Does anyone have experience with that? I'm a few days in and I'm wondering if I should just start over with a new vial because mine is ruined. I'm hoping it's a sturdier molecule than these websites make it out to be but it's my first time so I really don't know.

Thanks

 

[DesertDeadlifts666]

Not sure if destroyed but when reconstituting you aren’t supposed to shake the vial. More like inject the bac water and give the vial a gentle roll for a minute or so.

I’ve never reconstituted hgh but plenty of HCG. My process was to insert bac water into vial (maybe half of what I’d be inserting in total). Give it a gentle roll for like 20-30 seconds. Then inject the rest bac water and repeat the rolling process.

How long will the vial last? I’d just use it…

 

[Nattymatt]

Ive accidentallyinjected the water very quickly into the vial and had no probs. Ive never shaken a bottle tho

 

[BPrime2]

As everyone said, don't shake it. But, there is a good thread over on Meso from @Janoshik about how fragile peptides actually are (they aren't) that is good reading if you want some piece of mind.

I do my GH vials 12-24h before I'm going to use them, I just gently let the water run down the side of the vial, then put it in the fridge and let it dissolve at its own pace.

 

[caret912]

Thanks for the responses. There's only 3 days left on the vial so I'm going to finish it and be more careful with the next one.

When I try to let the water go in slowly it shoots out of the syringe into the vial without me pushing down the plunger. Is there any way to prevent this? I understand it's probably some vacuum effect from the different levels of pressure.

 

[BPrime2]

Thanks for the responses. There's only 3 days left on the vial so I'm going to finish it and be more careful with the next one.

When I try to let the water go in slowly it shoots out of the syringe into the vial without me pushing down the plunger. Is there any way to prevent this? I understand it's probably some vacuum effect from the different levels of pressure.

So my process is this:
3ml with 20-25g draw, 25-30g second pin
Take the small gauge second pin and pop it in the GH vial to equalize the pressure, then open your 3ml/draw pin combo and draw the appropriate MLs of Bac.
Remove the barrel from the back draw pin and put it on the pin that is stuck in the GH vial, then tilt and slowly add water.
Repeat as needed for doing multiple vials at once.

 

[username63741]

Thanks for the responses. There's only 3 days left on the vial so I'm going to finish it and be more careful with the next one.

When I try to let the water go in slowly it shoots out of the syringe into the vial without me pushing down the plunger. Is there any way to prevent this? I understand it's probably some vacuum effect from the different levels of pressure.

Contents of vial are under pressure. I use a 3ML syringe and fill it with just air then put it into the vial. It will auto pressurize. When it's equalized then I put in the water. This will create a positive pressure. Before removing water syringe, hold it above water line and let it fill with air to bring vial back to equalized pressure.

 

[caret912]

Contents of vial are under pressure. I use a 3ML syringe and fill it with just air then put it into the vial. It will auto pressurize. When it's equalized then I put in the water. This will create a positive pressure. Before removing water syringe, hold it above water line and let it fill with air to bring vial back to equalized pressure.

Beautiful, thanks.

 

[CaptainAmerica]

I’ve spoken to this before. Those vials are centrifuged… A little shake to mix all the powder into the Bac isn’t going to do anything. They aren’t as fragile as people think.

 

[BPrime2]

I’ve spoken to this before. Those vials are centrifuged… A little shake to mix all the powder into the Bac isn’t going to do anything. They aren’t as fragile as people think.

No, you're absolutely correct, however as someone who works with the general public in a medical capacity, what you and I understand as a light, safe shake and what someone else thinks is light can be and often are realms apart unless you can demonstrate in person.

 

[psauce]

No, you're absolutely correct, however as someone who works with the general public in a medical capacity, what you and I understand as a light, safe shake and what someone else thinks is light can be and often are realms apart unless you can demonstrate in person.

No person can exceed the forces generated by a centrifuge with manual shaking. This is very simple physics. Even the lamest benchtop centrifuge will accelerate specimens to 1000g. Most of them operate at upwards of 10000g. Peptides survive that process, with no quantitative loss, all the time.

We can approximate the forces generated by shaking pretty easily. Acceleration is change in velocity over change in time, so we need to know about how long it takes to shake a vial. I just did this sitting here (with my batin' hand to make sure it was extra vigorous) and got 30 shakes in 7.3 seconds. That's 0.24 s/shake. The maximum acceleration possible is essentially the same as how one would model an elastic collision... incident velocity is equal in sign but opposite in direction as resultant velocity. That makes a = 2v / dt.

In order to reach the lame-ass, barely working centrifuge acceleration of 1000g, one would have to be able to move his hand at 1176 m/s, or just excess of 2600 miles per hour. For context, that's 3-10 times as fast as the range of typical muzzle velocities for modern guns. It also means that it would take 26,000 miles per hour to reach the operating settings that most people use, day in and day out, to precipitate peptides.

The actual number for hand speed, assuming you move the vial about 5 inches, or approximately 1/8th of a meter, is more like 1 m/s. That makes the acceleration experience about three orders of magnitude off from what we know, for sure, peptides survive.

TLDR - Fragile peptides are a lie that serves people who sell bad gear.

 

[BPrime2]

TLDR - Fragile peptides are a lie that serves people who sell bad gear.

No one is saying in lyophilized form that shaking is bad, or at least I'm not, I have always worked under the impression that once you've reconstituted the peptide and put in into solution, there can be impact damage potentially, depending on the protein.
I will admit that is working knowledge from back when around when Jins first came on the scene so it's entirely probable that as we've gotten better at synthesis, it's not a problem anymore.

Edit: old knowledge is old and has been updated, never stop learning bros

https://thinksteroids.com/community/threads/does-shaking-hurt-hgh.134401748/