If you look at graphs below the Jano report, you can see the chromatogram that goes into making that calculation. If you look at the first chart, you see there's some minor noise around the 0.5-1.0 minute mark. It dips below the zero line, which means it fluoresces... you shine a light on it and it actually glows. That means it's probably methanol, ethanol, or acetic acid... weakly fluorescent compounds when exposed to UV and common solvents. That's a good sign and lets you know your column is working right and everything is flowing property to the detector. It's also a first indicator that the detector is working at the right sensitivity.Also, I was checking the Jano HPLC report for melanotan 1.
It confirms the quantity, but doesn't provide the purity.
Is there any reason for that?
Do you guys intend to get updated mt1 testing results including percentage purity?
I have low tolerance for mt2 so trying to find reliable tested mt1.
Very informative post. Not who you responds to but thx for sharing.If you look at graphs below the Jano report, you can see the chromatogram that goes into making that calculation. If you look at the first chart, you see there's some minor noise around the 0.5-1.0 minute mark. It dips below the zero line, which means it fluoresces... you shine a light on it and it actually glows. That means it's probably methanol, ethanol, or acetic acid... weakly fluorescent compounds when exposed to UV and common solvents. That's a good sign and lets you know your column is working right and everything is flowing property to the detector. It's also a first indicator that the detector is working at the right sensitivity.
The major peak occurs at what looks like 2.743 minutes. That corresponds to the analyte -- the melanotan. You see that it goes almost vertically up and has a long drop off. In this type of analysis (HPLC with an absorbance detector), a peak should ideally be perfectly symmetrical and "normal" in the statistical sense. A Gaussian peak. All this means is that there was too much injected into the system for analysis. Not horrible but not ideal. However, in THIS case, it's actually good -- by injecting too much, you actually exaggerate the amount of any contaminant that may be in the sample. In the time window that corresponds to the detector looking at what's coming off of the column, there's no other peak. That means anything that comes off the column comes off at about the same time as the MT1. So the two possibilities are: (1) there's no major contaminant anywhere, OR (2) the amount of contaminant is small enough that the MT1 swamps it out. So that passes the first test... the sample is overwhelmingly one compound and any impurity is limited in size.
The other graphs give you an idea of whether there's a contaminant "hiding" in with the MT1 when the sample reaches the detector, but is swamped out. In the second chart, you see there's one big spike right before the more roller coaster looking peak. That means there's a highly concentrated compound that comes into the detector right before the melanotan 1. I haven't ever looked at this type of data for this specific compound, but to the extent there is a contaminant, my guess is that it's there. The important thing to ask yourself: is the area under that spike really huge? It's not... it's a very narrow spike. That's pretty much all we can say in terms of possible contaminant.
If you look at the one labeled NMR, D2O, you can see the molecule of MT1. The highlighted portions correspond to the structure of the MT1 molecule that can be attributed to the various signals from the NMR. If you look, you see two molecules... one of them is actually acetic acid (CH3COOH), which matches our initial guess based on the UV fluorescence. The computer doesn't tell us whether there are NMR peaks that aren't attributable to MT1, and it would take a decent chunk of time to figure that out without having a known, pure NMR spectrum of MT1. However, you can see that each peak has a label attached, and that label has a number. That number corresponds to a part of the MT1 molecule. I don't see many a large peak that doesn't have a label -- one that doesn't correspond to either MT1 or acetic acid. That puts a hard upper limit on what else could be hiding away in with the MT1.
So while you don't have a percent number, you actually have much better data on exactly what might be in there down to the functional group of the potential contaminant. Nothing is perfectly pure, so there is sure to be a little bit of something, but it's not present in a huge amount.
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That means there's a highly concentrated compound that comes into the detector right before the melanotan 1. I haven't ever looked at this type of data for this specific compound, but to the extent there is a contaminant, my guess is that it's there. The important thing to ask yourself: is the area under that spike really huge? It's not... it's a very narrow spike. That's pretty much all we can say in terms of possible contaminant.
I don’t know enough about the particular procedure, but that seems like a rational guess.Could this be the acetic acid?
I understand this is a counter ion that's formed during melanotan synthesis?
How is your experience with these specific peptides so far?Just received my first order from xpeptides. Shipping was less than 4 days from time order was placed to PNW. Ordered 3 vials GHRP-2 and 3 vials modGRF. Packing was very discreet. Pucks looked much nicer than the supplier I had been using previously. Will post update after using the peptides for a while but as long as everything works as expected this will be my go to supplier for peptides in the future.
They are good to go. I’ve ordered multiple times from xpeptides since my initial order and have had no issues. I’ve used these same peptides from multiple other sources in the past and xpeptides stuff is as good if not better that any other source I’ve ordered from.How is your experience with these specific peptides so far?
To be more specific on the peptides, the hunger you get from ghrp-2 can be pretty intense when first starting or not having taken in a while but overall is very manageable. Using the 2 in conjunction with each other I find improved sleep quality, a fuller feeling in my muscle mass, a noticeable increase in recovery from workouts, faster hair and nail growth( this one is not necessarily a positive but a good sign that the products are legit and working as intended), and increased flexibility. Only con to this peptide combo that I have noticed is slight lethargy mid morning which is pretty common with GH and GH stimulating peptides.How is your experience with these specific peptides so far?
This is awesome feedback. I appreciate this. How much of each were you taking?To be more specific on the peptides, the hunger you get from ghrp-2 can be pretty intense when first starting or not having taken in a while but overall is very manageable. Using the 2 in conjunction with each other I find improved sleep quality, a fuller feeling in my muscle mass, a noticeable increase in recovery from workouts, faster hair and nail growth( this one is not necessarily a positive but a good sign that the products are legit and working as intended), and increased flexibility. Only con to this peptide combo that I have noticed is slight lethargy mid morning which is pretty common with GH and GH stimulating peptides.
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